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Promoter tagging in banana

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dc.contributor.author Remy, Serge
dc.contributor.author Coemans, Bert
dc.contributor.author Buysse, Sofie
dc.contributor.author Möller-Nielsen, Nina
dc.contributor.author Santos Ordoñez, Efren German
dc.contributor.author De Weerdt, Gerrit
dc.contributor.author Swennen, Rony
dc.contributor.author Sagi, Laszlo
dc.date.accessioned 2009-04-04
dc.date.available 2009-04-04
dc.date.issued 2002-09
dc.identifier.citation Remy S., Coemans B., Buysse S., Möller-Nielsen N., Santos E., De Weerdt G., Swennen R. and Sági L., 2002. Promoter tagging in banana. 3rd International Symposium on Molecular and Cellular Biology of Bananas. Leuven, Belgium, 9-11 September 2002. 23-24. Abstract. en
dc.identifier.uri http://www.dspace.espol.edu.ec/handle/123456789/4827
dc.description.abstract For the isolation of promoters with specific expression patterns a trapping technology has been assembled for banana, which allows in planta characterization of candidate promoters without a priori isolation of the corresponding genes. Detection and quantitation of luciferase expression was optimized in transgenic banana for capturing images by an ultrasensitive CCD camera. Time course and stability studies of luciferase expression were performed to determine time points for early detection. A promoterless luciferase reporter gene located next to a T-DNA border region has then been introduced to embryogenic cell suspensions of different banana cultivars. Screening for luciferase activation has been performed without induction as well as via various induction treatments during different steps of in vitro regeneration. Two to three months after transformation, screening of approximately 24,000 transgenic colonies revealed 16 candidates (0.07%) with constitutive expression. Screening of 12,000 colonies with the SAR (systemic acquired resistance) activator Bion® resulted in the identification of six (0.05%) inducible candidates. In parallel, out of 774 proliferating cultures (4-8 months after transformation) four (0.52%) exerted constitutive expression comparable to the CaMV35S promoter whereas among 157 differentiated in vitro plants (8-12 months after transformation) activation was observed in eight (5.1%) individuals. Further screening of colonies, proliferating cultures and in vitro plants has been performed under temperature, osmotic and aluminium stress conditions as well as after treatment with paraquat. Up to now, all candidates have been propagated for plant regeneration and molecular analysis. Regions upstream to the promoter tags have been cloned by PCR techniques and sequenced. en
dc.description.sponsorship Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven. en
dc.language.iso eng en
dc.publisher 3rd International Symposium on Molecular and Cellular Biology of Bananas en
dc.rights openAccess
dc.title Promoter tagging in banana en
dc.type Presentation en

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