Ingeniería de biorreactores para mejorar la biosíntesis de violaceína a partir de Pseudoalteromonas luteoviolacea

Abstract

CONDITION FOR PUBLICATION OF PROJECT. Cancer is one of the leading causes of death worldwide. In response to this problem, alternative treatments such as biopigments have been investigated, including violacein, which has demonstrated antitumor properties in animal trials. However, its limited production has hindered its advancement to human clinical trials. This project seeks to optimize key parameters that allow for higher concentrations of violacein, significantly reducing raw material costs. For the development of the project, an egg albumin decontamination protocol was developed using UV light, which guaranteed selectivity for the Pseudoalteromonas luteoviolacea bacteria. Culture media were then prepared, where assays were performed in 500 ml Kitasatos, varying temperatures, pH, agitation, and aeration, concluding with an assay in a 2000 ml Kitasato. The amount of protein in the albumin was quantified, demonstrating that a total exposure of 15 minutes to UV light degrades 7.51% of the protein. Furthermore, it was determined that a higher violacein concentration is achieved at 27°C, pH 6.5, low aeration, and no agitation. These findings make it possible to initiate controlled and sustainable violacein production, allowing for entry into a new industrial sector. Keywords: Process escalation, egg albumin, key parameters, substrate decontamination