Estudio comparativo de actividad lipolítica en cepas bacterianas mesófilas y antárticas para biorremediación de grasas

Abstract

CONDITION FOR PUBLICATION OF PROJECT. The accumulation of fats and oils in drainage systems leads to obstructions and environmental pollution, making sustainable alternatives for their degradation necessary. This study aims to compare the lipolytic potential of mesophilic bacterial strains and consortia isolated from urban grease traps with psychrophilic Antarctic bacteria optimized for bioremediation, under the hypothesis that microbial synergy enhances degradative activity. Bacterial strains were isolated and activated, followed by qualitative assays with rhodamine B and acid–base titrations to quantify lipolytic activity. Subsequently, strain compatibility was evaluated through antagonism tests, and a bacterial consortium (1:1:1 ratio) was formulated and tested in LB medium supplemented with olive oil. The results showed that the mesophilic consortium exhibited higher lipolytic activity than individual strains, even surpassing Antarctic bacteria inoculated under their optimal conditions. Therefore, it is concluded that combining native strains with high affinity for lipid substrates represents a promising strategy for the bioremediation of oily wastes, with strong potential for application in real-world environments. Keywords: lipolytic bacteria, microbial consortium, bioremediation.